The samples were treated with or without CPM in the presence of various concentrations of 1,4NQ. Results show that neither activation nor inhibition effects of 1,4NQ could be removed by re-spin wash in the absence of CPM;

• 用经CPM预处理过和没有处理过的样品与1 ,4NQ温育后离心清洗 ,发现未经CPM处理的样品经激活和抑制浓度的1 ,4NQ温育后 ,离心清洗不能消除1 ,4NQ对RyR1的激活和抑制。