The running buffer consisted of 30 mmol/L sodium tetraborate(pH 9.9) contanining 15% isopropanol shows,the best separation of amino acids derivatized with DNFB.The migration time is 30 min and it appears seventeen peaks.

• 以浓度为30 mmol/L、pH值9.；9的硼砂溶液含15%25异丙醇为运行缓冲液；分离氨基酸DNFB衍生物效果最佳；分离时间30 m in；出峰17个。