The results showed that eaeA gene was correctly inserted into the pET-28a(+) vector, and the recombinant expression plasmids (named pET-eaeA) were constructed successfully.

• 将经质粒PCR鉴定和双酶切鉴定为阳性的转化子测序作进一步鉴定，成功构建了重组表达质粒pET-eaeA。