The results showed that 92.5% of 30mM COBE was reduced to (R)-CHBE with 99% e.e. value by 100g/L the recombinant cells at optimal conditions.

• 优化其还原反应条件；结果表明；100g/L重组菌在32℃；以150mM的葡萄糖为辅助底物的缓?_溶液(pH6.；0)中能将30mM的4-氯乙酰乙酸乙酯(COBE)还原为(R)-CHBE；产物得率为92