The results showed that 92.5% of 30mM COBE was reduced to (R)-CHBE with 99% e.e. value by 100g/L the recombinant cells at optimal conditions.
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优化其还原反应条件;结果表明;100g/L重组菌在32℃;以150mM的葡萄糖为辅助底物的缓?_溶液(pH6.;0)中能将30mM的4-氯乙酰乙酸乙酯(COBE)还原为(R)-CHBE;产物得率为92
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