The results show that cold methanol extraction method has the best reproducibility;highest signal intensities and can simultaneously detect more than 20 intracellular metabolites.

• 结果显示，冰甲醇抽提的样品在电喷雾质谱实验中表现出良好的重复性和最高的峰强度，并能够同时检测到超过20种不同的胞内代谢物。