The results of cytological observation showed that the abnormal feature of pollen development appeared at binucleate stage.The starch was failed to accumulated in pollen and lead to mature sterile.

• 细胞学观察T0代转化植株花粉从单核到成熟发育过程，结果表明花粉育性下降的原因主要是由于二核期花粉发育淀粉粒累积受阻所致。