The results demonstrated that pET-28a(+)ND-1sc(Fv)2 gene was constructed and expressed successfully in E.

• 结果表明成功构建了表达载体pET-28a(+)ND-1sc(Fv)2,并在大肠杆菌中获得高效表达,其表达产物以不溶性包涵体形式存在。