The recombination plasmid was transiently transfected into COS7 cells and the expression of the fusion protein was identified by RT PCR, immunofluorescent assay and sandwich ELISA.

• 以其瞬时转染COS7细胞 ,应用RT PCR、细胞免疫荧光技术 ,及夹心ELISA检测融合蛋白的表达。