The recombinant virus CAV2-TSOL18m was obtained and the classical cytopathic effects(CPE) of CAV-2 could be observed .

• Asc I+Cla I双酶切pCAV2-TSOL18m释放重组基因组，转染DK细胞，获得了重组病毒CAV2-TSOL18m。 该重组病毒在DK细胞上能产生典型的腺病毒细胞病变。