The recombinant plsmids were transfected into COS-7 cells respectively and to test the expressed target proteins by RT-PCR, ELISA and dot blotting.

• pcDNA3.；1(+)、pcDNA/Ag85B、pcDNA/MPT64和pcDNA/AM转染COS-7细胞，用RT-PCR、ELISA和斑点印迹的方法鉴定目的基因的表达；