The recombinant plasmid pAd-K14-E6/E7-polA was successfully constructed and confirmed by restriction endonuclease digestion and sequencing.

• 通过同源重组的方法构建了腺病毒pAd-K14-E6/E7-polA载体,经酶切和测序鉴定该质粒构建成功。