The recombinant adenovirus vector, pAdCMV-IL3-Endo, was cotransfected into 293 cells together with pBHG10 by lipofectamine, and recombinant adenovirus, which named Ad-ILs-Endo, were generated by homologous recombination.

• 然后用脂质体法将上述穿梭质粒与质粒pBHG10共转染293细胞，经同源重组获得复制缺陷型重组腺病毒Ad-IL_3-Endo。