The recombinant K99 and F41 fimbriae antigens expressed by E. coli RRI (pMG611) strain had been purified by heat treatment and then by Sephadex G-100 filtratiion.

• 本文利用加热搅拌及Sephadex G-100凝胶过滤方法,从双价重组工程菌RRI(pMG611)中分离了重组K99和F41菌毛抗原。