The protoplast DES mutagenesis for more increasing arachidonic acid was studied on Mortierella alpina strain. The experiments showed that the high mutants could by obtained by DES to the protoplasts with 3 minutes.

• 采用化学诱变剂硫酸二乙酯 (DES)对由高山被孢霉出发菌株M 3 18制备的原生质体进行诱变育种 .