The promoter that RNA polymerase III recognize are U6 promoter or H1 promoter, therefore the key step of plasmid-based RNA interference technology is cloning the RNA interference target sequence to downstream of U6 or H1 promoters.

• RNA聚合酶识别的是U6或H1启动子，所以将RNA干扰序列构建到这些启动子的下游是以质粒载体为基础的RNA干扰技术的关键步骤。