The products of PCR werenamed Sa and Sb, of 2.1Kb and 2.3Kb respectively. Sa was inserted in EcoR I andKpn I sites after Sb was cloned in KpnI and PstI multiple cloning sites of the samepUC18 plasmid.
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释义
1Kb和2.;3Kb两个片段,经酶切鉴定后,先后插入到pUC18质粒载体上的EcoRI和PstI多克隆位点上,构建了重组质粒pUC-S。
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