The procedure involved extraction of TSNA from tobacco by sonication,elu-tion of extract in a Kieselguhr extraction column ,concentration of elute and determination of TSNA by gas chromatography with a N-P detector.

• 主要过程包括烟样的超声波萃取,萃取液在硅藻土层析柱上层析,洗脱液的浓缩及应用气相色谱-NPD进行检测。