The prion protein(PRNP) genes of 25 Amur tigers were amplified by PCR using primers designed from mammalian PRNP. These PCR products contain 402 bp encoding 134 amino acids,and have a homology of 99.67%.

• 根据已报道的哺乳动物朊病毒基因序列设计引物；采用PCR方法扩增了25只东北虎的朊病毒基因；克隆、测序及序列分析表明；所得到的东北虎朊病毒基因片段为402bp；编码134个氨基酸的前体蛋白；核苷酸序列同源性为99.；67%25。