The plasmid was made? by P. Ghosh, who solved the structure of colicin Ia then years ago (published in Nature). A promoter and SD sequence was introduced into the plasmid.

• 回答:如果克隆序列有翻译开始信号，是可以用来表达的，但是从该论文公布的克隆序列看，没有由方舟子于2006-1-12,02:18:51: