The plasmid pTWIN1/Sj-F1 was transformed into E. coli ER2566. The soluble recombinant fusion protein (rSj-F1/intein2) was expressed in E. coli under low IPTG concentration and low temperature, then analyzed by SDS-PAGE and Western blot.

• 将pTWIN1/Sj-F1质粒转化大肠杆菌ER2566；在低温和低IPTG浓度下诱导表达可溶性重组融合蛋白(rSj-F1/intein2)；并经SDS-聚丙烯酰胺凝胶电泳(SDS-PAGE)和蛋白质印迹(Western blot)分析鉴定.