The plasma and brain tissue were added with acetonitrile-0.1mol/L perchloric acid, then vortex-mixed and centrifuged, finally the clear supernatant was analyzed with HPLC.

• 血浆或脑组织解冻后，用0.；1mol/L的高氯酸和乙腈进行提取，涡旋离心后取上清进样。