The optimum conditions were glucose,1 2%,(NH 4) 2SO 4,0 6%,and yeast extract 0 5%,the time and quantity of adding disodium cis epoxysuccinate was 32h and 3 58%,respectively.

• 正交优化试验的最佳培养基和培养时间为 :葡萄糖 1 2%25 ,硫酸铵 0 6%25 ,酵母膏0 5%25 ; 顺式环氧琥珀酸二钠投加时间为 30h ,投加量为 3 5 8%25 ;