The optimal conditions were as follows:pH of the buffer for immobilization being 7.0,the size of immobilized cells 2.5 mm,the reaction solvent hexane,co-substrates 1-hexanol and the initial concentrations of COBE 54.7 mmol/L,respectively.

• 固定化时所用缓冲液的pH值为7.；0时和固定化细胞颗粒平均直径为2