The oligosaccharides were tagged with the charged fluorophore 8-aminonaphthalene-1,3,6-trisulfonate(ANTS), Saccharide-ANTS adducts were then separated from one another by electrophoresis on a 32% acrylamide, 2.4% bisacrylamide polyacrylamide gel.

• 寡糖首先与8-氨基萘基-1；3；6-三磺酸(ANTS)反应记；然后；ANTS标记的寡糖通过在32%25丙烯酰胺-2.；4%25双丙烯酰胺组成的分离胶上电泳从而得以相互分离。