The novel vector pET-28a( + ) ND-lsc(Fv)2 was transformed into E. coli BL21. Condition for optimal expression was adjusted.

• 将质粒pMD18一几nke卜ND一1 scFv进行SalF凡ndln双酶切，产物片段hnker一ND一1 seFv与经SalFHindlll双酶切消化的pET一sa(+)ND一1 seFv连接，转化感受态E.