The maximum reacting dilutions of crude sap (TMV) are 1:5 120 and CMV are 1:2 560.The coparison of three blocking systems (TBST, TBST+3%BSA, TBST+3%Casein) in TMV and CMV detection showed that both TBST+3%BSA and TBST+3% Casein are better than TBST.

• 检测感染叶片粗汁液的最高稀释度TMV为1:5 120; CMV为1:2560.；利用3种不同封闭系统[TBST；TBST+3%25牛血清白蛋白(3%25BAs)；TBST+3%25酪蛋白(3%25Casein)]检测；比较结果表明;