The mature DCs were co-cultured with autologous T cells to obtain antigen specific CTL (Ag-CTL) and antigen non-specific CTL (non Ag-CTL). The phenotypes of Ag-CTL were analyzed by flow cytometry.

• 成熟DCs与自体T细胞共育,分别获得Ag-CTL及non-Ag-CTL,流式细胞仪检测Ag-CTL细胞表型的变化;