The ladder experiments showed: the best PCR amplified system of the crested ibis is as follows: Primer(10pmol/ul)lul, Taq enzyme(lU/ul)1.5ul, dNTP(2.5mM)2.0ul, Mg2+(25mM)2.5ul, teplate DNAlOng, TH2O17ul, and final volume is 25ul.

• 本试验对各种影响因素进行了梯度筛选，得到朱?最佳的PCR扩增体系为：引物(10pmol/ul)1ul、TaqDNA聚合酶(1U/ul)1.；5ul、dNTP(2