The karyotype of toad,Bufo raddei,was analysed by the technique of leukocytes culture in vitro and compared with karyotype of Bufo bufo gargarizans.

• 摘要 采用淋巴细胞培养的方法，分析了花背蟾蜍的核型，并与中华大蟾蜍进行了比较。