The isozyme proteins of est 1, 2 and est 3, 4, in esterase isozyme loci Est A and Est B, were purified and segregated from mycelia of Agaricus bisporus with polyacrylamide slab gel electrophoresis (PAGE).

• 采用PAGE法分离提纯双孢蘑菇菌丝酯酶标记位点Est A与B的同工酶蛋白est 1,2和est 3,4。