The interaction of bovine serum albumin(BSA) with acid brown SR(ASR) was investigated by spectrophotometric method.Based on the spectrum of ASR, it has two structures in neutral aqueous solution.

• 采用分光光度法研究了酸性棕SR(ASR)在不同酸度条件下的吸收光谱；并研究了其与牛血清白蛋白(BSA)的结合反应；探讨了结合反应的机理；考察了表面活性剂对结合反应的影响.；实验证明:ASR存在着两种平衡结构;