The influences of PAN on the total protein concentration were measured with coomassie brilliant blue and the effects of PAN on the expression of ERK1/2 were studied by western blotting analysis.

• 考马斯亮兰蛋白测定法检测PAN 对细胞总蛋白浓度的影响; Western Blotting 法观察PAN 对细胞外信号调节激酶(ERK1/2)蛋白表达的影响。