The high yield of lysozyme activity obtained at high starting concentration illustrates the workability of this method for high concentration protein refolding.

• 复性溶菌酶与天然溶菌酶色谱保留体积的差异揭示出折叠过程中无活性蛋白质聚集体的存在及其向复性蛋白质转化的机制；