The heavy chains variable region gene (VH) of monoclonal antibody against beet necrotic yellow vein virus (BNYVV) was amplified from total DNA extracted from anti-BNYVV hybridoma cells by PCR.

• 用PCR方法以分泌抗甜菜坏死黄脉病毒（BNYVV）单克隆抗体的杂交瘤细胞的基因组为模板，扩增了编码BNYVV单抗的重链可变区（VH）基因。