The green shoot regeneration frequencies were 0.37% with PPA-based one-step medium, 0.21% with M8 medium and 0.14% with SH medium. It indicated that the PAA based one-step medium was most effective in the anther culture of indica genotypes.

• 其中，一次成苗培养基、M_8培养基和SH培养基花培绿苗率分别为0.；37%25，0