The genomic DNA was isolated and purified with the improved CTAB methods. The sequences of 16S rRNA gene were amplified by TD-PCR with the bacterium universal primers and the purified PCR products were directly sequenced.

• 菌株经纯化培养；以改良CTAB法提取总DNA；采用细菌16S rRNA通用引物、TD-PCR方法(touchdown-PCR)进行16S rRNA基因序列扩增；PCR产物纯化后直接进行序列测定；序列经人工校对后用Clustal X进行比对分析；最后用MEGA3.；1软件构建系统发育树。