The fragment containing UBE gene was inserted into pGEX-4T-2 expressive vector, and induced by IPTG. Molecular weight of ubiquitin fusion protein was about 40 kD by checking with SDS polyacrylamide gel electrophoresis.

• 将烟夜蛾的UBE基因克隆到原核表达载体pGEX-4T-2上,经IPTG诱导,SDS-PAGE电泳检测出约40kD的融合蛋白。