The expression plasmid pSV-F was constructed by inserting the F gene into the pVAX1 vector, and transfected into the cultured COS 7 cell line via liposomes.

• 将HeB02株F基因插入真核表达载体pVAX1中,构建了真核表达质粒pSV-F,通过脂质体转染COS-7细胞,SDS-PAGE分析可见表达的特异蛋白条带;