The expression of zebrafish TS protein was induced by IPTG in E. coli BL21 (DE3). High level expression of zebrafish TS was found at 1mM IPTG condition after incubation for 3h.

• 将斑马鱼TS蛋白完整编码序列连接到pET-28a表达载体上,构建表达载体pET-28/Z-TS,转化进大肠杆菌BL21(DE3)进行IPTG诱导性融合表达,实验表明在1mM的IPTG浓度条件下,诱导表达3h,斑马鱼TS融合蛋白表达量达到比较高的水平。