The expression of GDF-9 was higher in group A than that of group B. The weak positive rate,positive rate and strong positive rate in group C was 28.1%,53.1% and 18.8% while that in group D was 61.5%,30.8% and 7.7%, respectively.

• 卵泡培养后共收集卵母细胞58个,C组32个,染色强度为+~+++的卵泡数分别为9(28·1%25)、17(53·1%25)、6(18·8%25)个;