The eukaryotic expression plasmid pcD85B was constructed. The recombinantplsmid was transfected into COS-7 cells and the expressed target protein was tested byRT-PCR, ELISA and dot blotting.

• pcD3.；1(+)、pcD85B、转染 COS-7 细胞；用 RT-PCR、ELISA 和斑点印迹的方 法鉴定目的基因的表达;