The entire sequence of LT gene was amplified by PCR from Escherichia coli 216,using a specific primer based on the reported E. coli heat-labile enterotoxin gene sequence.

• 根据已报道的大肠杆菌不耐热肠毒素（LT）的基因序列设计引物，用PCR方法从Escherichia coli 216株基因组中扩增出LT基因的全序列。