The determination was carried out under acidic condition, using CHC13 as a solvent, Dioctyl Sodium Sulfosuccinate as a volumetric solution, and Dimethyl Yellow-Methylene Blue as mixed indicator.

• 三氯甲烷为溶剂，磺酸钠基丁二酸二辛酯溶液为滴定液，用二甲基黄-亚甲蓝混合指示液，在酸性条件下进行滴定，以测定苦参碱含量。