The content of total alkaloid in the seed of Peganum harmala L. was determined by bro-mophenol blue colorimetry.

• 以溴酚蓝作为测试用酸性染料，在总生物碱与其形成稳定络合物时间范围内，利用比色法制定了骆驼蓬种子中总生物碱的含量。