The conjugates, whose absorbance peak shifted ,and was between the absorbance peaks of haptens and carrier proteins, were confirmed by UV-scanning.

• 最终交联结合物进行紫外扫描，结果表明，结合抗原最大吸收峰发生了漂移，介于小分子半抗原和载体蛋白之间，表明交联成功。