The conditions of establishing the high regeneration system of Zoysiagrass were studied, the genes of IPT and CBF1 were transformed into the calli using microprojectile bombardment and Agrobacterium tumefaciens transformation.

• 我们在建立了高效的结缕草组培再生体系的基础之上,通过基因枪法和农杆菌介导法转化CBF_1和IPT基因,获得转化CBF_1基因的植株,为培育新品种打下基础。