The competent Agrobacterium tumefacien was transformed by rice expression vector p13W4-CTB and pCWUN respectively.

• 水稻表达载体P13w4一cTB和pcWIJN分别转化感受态根瘤土壤杆菌，经过PcR筛选和酶切鉴定，获得根瘤土壤杆菌转化子。