The capped viral genomic RNA was transcribed in vitro,mixed with liposome and transfected into MARC-145 cells,and an infectious virus(designated rV68) was rescued.

• 通过体外转录获得病毒基因组RNA,将RNA与脂质体混合后直接转染MARC-145细胞,获得拯救病毒(rV68)。