The cDNA of BcpLH gene was cloned into a His-fusion expression vector pET-28a (+) and was induced to express in E. colt strain BL21(DE3).

• 在含有His标记序列的原核表达载体pET28-a(+)上插入Bc-pLH基因的cDNA,在大肠杆菌BL21(DE3)中诱导表达出了特异性蛋白,并免疫大白兔制备出高效价的抗血清;