The cDNA encoding the extracellular domain of human costimulatory molecule B7 1/CD80 was cloned from human peripheral blood monocytes by using RT PCR method and sequenced by Sanger chain termination method.

• 用RT PCR法从人外周血单核细胞克隆了编码共刺激分子B7 1 /CD80胞外区的cDNA ；并用Sanger链终止法进行测序 .